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ccl21 elisa  (R&D Systems)


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    R&D Systems ccl21 elisa
    Fig. 5 Upregulation of stromal cell activation genes precedes B cell infiltration after intranasal infection. Mice were infected with MHV-A59 IN with 50,000 PFU and olfactory bulbs were harvested 1, 3, 5, and 7 dpi. Gene expression of indicated genes, including viral N gene, stromal cell genes Pdpn, Fap, Ccl19, <t>Ccl21,</t> Ltbr, stromal cell activating Ltb, as well as B cell markers Ms4a1 and Ighd, relative to Gapdh expression is shown as mean of each group ± SD. Statistical significance between naïve and various timepoints determined by one-way ANOVA and Dunnett’s multiple comparison test (*p < 0.05, **p < 0.01, ***p < 0.001)
    Ccl21 Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ccl21 elisa - by Bioz Stars, 2026-08
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    1) Product Images from "B cells are not drivers of stromal cell activation during acute CNS infection."

    Article Title: B cells are not drivers of stromal cell activation during acute CNS infection.

    Journal: Journal of neuroinflammation

    doi: 10.1186/s12974-025-03491-7

    Fig. 5 Upregulation of stromal cell activation genes precedes B cell infiltration after intranasal infection. Mice were infected with MHV-A59 IN with 50,000 PFU and olfactory bulbs were harvested 1, 3, 5, and 7 dpi. Gene expression of indicated genes, including viral N gene, stromal cell genes Pdpn, Fap, Ccl19, Ccl21, Ltbr, stromal cell activating Ltb, as well as B cell markers Ms4a1 and Ighd, relative to Gapdh expression is shown as mean of each group ± SD. Statistical significance between naïve and various timepoints determined by one-way ANOVA and Dunnett’s multiple comparison test (*p < 0.05, **p < 0.01, ***p < 0.001)
    Figure Legend Snippet: Fig. 5 Upregulation of stromal cell activation genes precedes B cell infiltration after intranasal infection. Mice were infected with MHV-A59 IN with 50,000 PFU and olfactory bulbs were harvested 1, 3, 5, and 7 dpi. Gene expression of indicated genes, including viral N gene, stromal cell genes Pdpn, Fap, Ccl19, Ccl21, Ltbr, stromal cell activating Ltb, as well as B cell markers Ms4a1 and Ighd, relative to Gapdh expression is shown as mean of each group ± SD. Statistical significance between naïve and various timepoints determined by one-way ANOVA and Dunnett’s multiple comparison test (*p < 0.05, **p < 0.01, ***p < 0.001)

    Techniques Used: Activation Assay, Infection, Gene Expression, Expressing, Comparison



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    Aging skews the peritoneal secretome toward a proinflammatory microenvironment. An abundance of proinflammatory mediators in the peritoneal fluid of naïve 3- and 21-mo-old female (♀) and male (♂) C57Bl6 mice were analyzed using a cytokine array. Fluids from 3 mice per age group for each sex were pooled, in which n = 1. Heatmaps depict the fold change in (A) chemokines/chemoattractants, growth/survival factors, and soluble adhesion molecules, with (D) cytokines of aged mice relative to young mice for each sex. Analytes with <3-fold change are represented by white squares. Quantification of (B) <t>CCL21</t> and (C) CXCL13 in the peritoneal lavage fluid of 3-, 18-, and 21-mo-old female and male wild-type mice by ELISA. Analysis of variance showed a significant effect of age ( P < 0.001) and sex ( P < 0.001) on the concentration of CCL21 in the peritoneal fluid and of age ( P < 0.001), but not sex, on the concentration of CXCL13. Data are presented as mean ± SEM using n = 4 to 5 per age group, from n = 1 independent experiment. * P < 0.05, ** P < 0.01, and *** P < 0.001 by Bonferroni multiple comparison posttest.
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    Fig. 5 Upregulation of stromal cell activation genes precedes B cell infiltration after intranasal infection. Mice were infected with MHV-A59 IN with 50,000 PFU and olfactory bulbs were harvested 1, 3, 5, and 7 dpi. Gene expression of indicated genes, including viral N gene, stromal cell genes Pdpn, Fap, Ccl19, <t>Ccl21,</t> Ltbr, stromal cell activating Ltb, as well as B cell markers Ms4a1 and Ighd, relative to Gapdh expression is shown as mean of each group ± SD. Statistical significance between naïve and various timepoints determined by one-way ANOVA and Dunnett’s multiple comparison test (*p < 0.05, **p < 0.01, ***p < 0.001)
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    Fig. 5 Upregulation of stromal cell activation genes precedes B cell infiltration after intranasal infection. Mice were infected with MHV-A59 IN with 50,000 PFU and olfactory bulbs were harvested 1, 3, 5, and 7 dpi. Gene expression of indicated genes, including viral N gene, stromal cell genes Pdpn, Fap, Ccl19, <t>Ccl21,</t> Ltbr, stromal cell activating Ltb, as well as B cell markers Ms4a1 and Ighd, relative to Gapdh expression is shown as mean of each group ± SD. Statistical significance between naïve and various timepoints determined by one-way ANOVA and Dunnett’s multiple comparison test (*p < 0.05, **p < 0.01, ***p < 0.001)
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    Fig. 5 Upregulation of stromal cell activation genes precedes B cell infiltration after intranasal infection. Mice were infected with MHV-A59 IN with 50,000 PFU and olfactory bulbs were harvested 1, 3, 5, and 7 dpi. Gene expression of indicated genes, including viral N gene, stromal cell genes Pdpn, Fap, Ccl19, <t>Ccl21,</t> Ltbr, stromal cell activating Ltb, as well as B cell markers Ms4a1 and Ighd, relative to Gapdh expression is shown as mean of each group ± SD. Statistical significance between naïve and various timepoints determined by one-way ANOVA and Dunnett’s multiple comparison test (*p < 0.05, **p < 0.01, ***p < 0.001)
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    Image Search Results


    Aging skews the peritoneal secretome toward a proinflammatory microenvironment. An abundance of proinflammatory mediators in the peritoneal fluid of naïve 3- and 21-mo-old female (♀) and male (♂) C57Bl6 mice were analyzed using a cytokine array. Fluids from 3 mice per age group for each sex were pooled, in which n = 1. Heatmaps depict the fold change in (A) chemokines/chemoattractants, growth/survival factors, and soluble adhesion molecules, with (D) cytokines of aged mice relative to young mice for each sex. Analytes with <3-fold change are represented by white squares. Quantification of (B) CCL21 and (C) CXCL13 in the peritoneal lavage fluid of 3-, 18-, and 21-mo-old female and male wild-type mice by ELISA. Analysis of variance showed a significant effect of age ( P < 0.001) and sex ( P < 0.001) on the concentration of CCL21 in the peritoneal fluid and of age ( P < 0.001), but not sex, on the concentration of CXCL13. Data are presented as mean ± SEM using n = 4 to 5 per age group, from n = 1 independent experiment. * P < 0.05, ** P < 0.01, and *** P < 0.001 by Bonferroni multiple comparison posttest.

    Journal: Journal of Leukocyte Biology

    Article Title: Aging modulates homeostatic leukocyte trafficking to the peritoneal cavity in a sex-specific manner

    doi: 10.1093/jleuko/qiad053

    Figure Lengend Snippet: Aging skews the peritoneal secretome toward a proinflammatory microenvironment. An abundance of proinflammatory mediators in the peritoneal fluid of naïve 3- and 21-mo-old female (♀) and male (♂) C57Bl6 mice were analyzed using a cytokine array. Fluids from 3 mice per age group for each sex were pooled, in which n = 1. Heatmaps depict the fold change in (A) chemokines/chemoattractants, growth/survival factors, and soluble adhesion molecules, with (D) cytokines of aged mice relative to young mice for each sex. Analytes with <3-fold change are represented by white squares. Quantification of (B) CCL21 and (C) CXCL13 in the peritoneal lavage fluid of 3-, 18-, and 21-mo-old female and male wild-type mice by ELISA. Analysis of variance showed a significant effect of age ( P < 0.001) and sex ( P < 0.001) on the concentration of CCL21 in the peritoneal fluid and of age ( P < 0.001), but not sex, on the concentration of CXCL13. Data are presented as mean ± SEM using n = 4 to 5 per age group, from n = 1 independent experiment. * P < 0.05, ** P < 0.01, and *** P < 0.001 by Bonferroni multiple comparison posttest.

    Article Snippet: The concentration of CCL21 (DY457) and CXCL13 (DY470) in the PLF and TNFα (DY410) and IL-6 (DY406) in the serum was quantified using enzyme-linked immunosorbent assay (ELISA) (all from Bio-Techne) according to the manufacturer's instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Comparison

    Fig. 5 Upregulation of stromal cell activation genes precedes B cell infiltration after intranasal infection. Mice were infected with MHV-A59 IN with 50,000 PFU and olfactory bulbs were harvested 1, 3, 5, and 7 dpi. Gene expression of indicated genes, including viral N gene, stromal cell genes Pdpn, Fap, Ccl19, Ccl21, Ltbr, stromal cell activating Ltb, as well as B cell markers Ms4a1 and Ighd, relative to Gapdh expression is shown as mean of each group ± SD. Statistical significance between naïve and various timepoints determined by one-way ANOVA and Dunnett’s multiple comparison test (*p < 0.05, **p < 0.01, ***p < 0.001)

    Journal: Journal of neuroinflammation

    Article Title: B cells are not drivers of stromal cell activation during acute CNS infection.

    doi: 10.1186/s12974-025-03491-7

    Figure Lengend Snippet: Fig. 5 Upregulation of stromal cell activation genes precedes B cell infiltration after intranasal infection. Mice were infected with MHV-A59 IN with 50,000 PFU and olfactory bulbs were harvested 1, 3, 5, and 7 dpi. Gene expression of indicated genes, including viral N gene, stromal cell genes Pdpn, Fap, Ccl19, Ccl21, Ltbr, stromal cell activating Ltb, as well as B cell markers Ms4a1 and Ighd, relative to Gapdh expression is shown as mean of each group ± SD. Statistical significance between naïve and various timepoints determined by one-way ANOVA and Dunnett’s multiple comparison test (*p < 0.05, **p < 0.01, ***p < 0.001)

    Article Snippet: CCL21 ELISA (Cat#DY457, RnD Systems) was performed according to manufacturer’s instructions.

    Techniques: Activation Assay, Infection, Gene Expression, Expressing, Comparison